{ "nid": "93", "details": "\x3cspan class=\"title\"\x3eNerveWorks: IV Relations\x3c/span\x3e\x3cbr/\x3e\n\x3cspan class=\"description\"\x3eThis is an advanced and somewhat difficult lab using the voltage clamp to record Na\x3csup\x3e+\x3c/sup\x3e current through voltage-gated Na channels. You generate a Current\x3ci\x3e vs. \x3c/i\x3eVoltage (I-V) relation for the Na\x3csup\x3e+\x3c/sup\x3e current and explore the factors that determine its U-shape. Specifically that depolarization has two effects on the Na\x3csup\x3e+\x3c/sup\x3e current amplitude: it makes it larger by opening more Na\x3csup\x3e+\x3c/sup\x3e channels and also makes it smaller by reducing driving force as the depolarization approaches the Nernst potential for Na\x3csup\x3e+\x3c/sup\x3e.You use your data to construct a plot of Na\x3csup\x3e+\x3c/sup\x3e conductance \x3ci\x3evs.\x3c/i\x3e voltage. After learning how to generate these plots you record voltage-gated K currents and make I-V and g-V plots for this current.\r\n\x3c/span\x3e\x3cbr/\x3e\n\x3cbr/\x3e\n\x3cstrong\x3eKey Concepts\x3c/strong\x3e: IV Relationships; Voltage clamp\x3cbr/\x3e\n\x3cstrong\x3eCourses\x3c/strong\x3e: Neurobiology\x3cbr/\x3e\n          \x3cbr/\x3e\x3ca href=\"/search_result_details/brief/93\" class=\"node_brief\"\x3eRead less\x3c/a\x3e        \x3cbr/\x3e  \x3c/span\x3e \x3c!-- description --\x3e\n" }